What Is the Function of a Microscope? By Jon Zamboni; Updated April 26, The microscope is one of the most important tools used in chemistry and biology.
The first set of lenses are the oculars, or eyepieces, that the viewer looks into; the second set of lenses are the objectives, the lenses closest to the object specimen.
Before purchasing or using a microscope, it is important to know the functions of each part. The eyepieces are the lenses at the top that the viewer looks through; they are usually Parts and function of microscope or 15X. To get the total magnification level, multiply the magnification of the objective used ex: Where the eyepieces are dropped in.
Also, they connect the eyepieces to the objective lenses. The bottom of the microscope—what the microscope stands on. Structural element that connects the head of the microscope to the base. The flat platform that supports the slides. Stage clips hold the slides in place. If your microscope has a mechanical stage, the slide is controlled by turning two knobs instead of having to move it manually.
One knob moves the slide left and right, the other moves it forward and backward. A steady light source volts in the US that shines up through the slide. Mirrors are sometimes used in lieu of a built-in light. If your microscope has a mirror, it is used to reflect light from an external light source up through the bottom of the stage.
This circular structure is where the different objective lenses are screwed in. To change the magnification power, simply rotate the turret. Usually you will find 3 or 4 objective lenses on a microscope.
The most common ones are 4X shortest lens10X, 40X and X longest lens. The higher power objectives starting from 40x are spring loaded. Spring loaded objective lenses will retract if the objective lens hits a slide, preventing damage to both the lens and the slide.
All quality microscopes have achromatic, parcentered, parfocal lenses. In addition, to get the greatest clarity at high levels of magnification, you will need a microscope with an Abbe condenser. Lenses are color coded and are interchangeable between microscopes if built to DIN standards.
This feature determines how far up the stage can go. Setting the rack stop is useful in preventing the slide from coming too far up and hitting the objective lens.
Normally, this adjustment is set at the factory, and changing the rack stop is only necessary if your slides are exceptionally thin and you are unable to focus the specimen at higher powers.
Condenser lenses focus the light that shines up through the slide, and are useful for attaining sharp images at magnifications of X and above. If the maximum power of your microscope is X, a stage mounted 0.
However, if your microscope goes to X or above, focusable condenser lens with an N. Most microscopes that go up to X come equipped with an Abbe condenser, which can be focused by moving it up and down. The Abbe condenser should be set closest to the slide at X, and moved further away as the magnification level gets lower.
The diaphragm or iris is located under the stage and is an apparatus that can be adjusted to vary the intensity, and size, of the cone of light that is projected through the slide.
As there is no set rule on which setting to use for a particular power, the setting depends on the transparency of the specimen and the degree of contrast you desire in your image. What to look for when purchasing a microscope: If you want an instrument that can provide you with crisp, high-quality images at high resolutions, stay away from microscopes with plastic components.
Instead, look for a microscope that has a metal body and all glass lenses. Make sure you purchase your precision instrument from a well-established dealer who will be around to help you with technical problems in case you have issues with your microscope.
Technical support is one simple phone call or email away.Microscope Parts and Functions A microscope is an instrument widely to magnify and resolve the image of an object that is otherwise invisible to naked eye.
For resolving the details of objects, which otherwise cannot be achieved by naked eye, a microscope is used. Compound Microscope Parts. A high power or compound microscope achieves higher levels of magnification than a stereo or low power microscope.
It is used to view smaller specimens such as cell structures which cannot be seen at lower levels of magnification. Essentially, a compound microscope consists of structural and optical components.
The microscope has been used in science to understand element, diseases and cells. In the science lab today we covered the basics on . Compound Microscope Parts A high power or compound microscope achieves higher levels of magnification than a stereo or low power microscope.
It is used to view smaller specimens such as cell structures which cannot be seen at lower levels of magnification. The microscope gets its name from the Greek words micro, meaning small, and skopion, meaning to see or look, and it literally is a machine for looking at small things.
A microscope may be used to look at the anatomy of small organisms such as insects, the fine structure of rocks and crystals, or individual cells. Stereo Microscope: A low power microscope or dissecting microscope with a separate eyepiece and objective lens for each eye.
These separate optical channels enable stereo or three-dimensional images of the specimen. See Compound Microscope. Sub-Stage: The parts of the microscope below the stage, including the illumination system.